Proteonano™ and Evosep Integration for Automated High-Throughput Proteomics Workflow
By integrating the Proteonano and Evosep platforms into a unified automated workflow, we enable exceptionally high throughput and robust reproducibility for large-scale proteomic studies. This solution provides critical technological support for mass spectrometry‒based proteomics at the scale of tens of thousands of samples.
The Evosep One is a high-throughput, highly stable, and ultra-low contamination liquid chromatography system designed for mass spectrometry-based proteomics research. Paired with the Evotip, which enables online desalting, the Evosep One enhances system stability.
Nanomics Biotech Proteonano ™ ultraplex proteomics platform enablesautomating steps up to enzymatic digestion or desaltingprior to mass spectrometry. Both Proteonano and Evosep are designed for large-scale proteomics studies, offering standard, high-throughput, and automated processes. Combining these platforms allows seamless transition of peptides into the Evotip for desalting, followed by automatic injection into the Evosep system for mass spectrometry.
The integration of the Proteonano and Evosep platforms into a unified automated workflow provides exceptional throughput and stability for large-scale proteomics studies, supporting mass spectrometry-based proteomics for cohort sizes of up to thousands.
Conclusion
This study integrates the Proteonano™ Basic Suite and the Evosep one platform for high-throughput proteomics, achieving automated high-throughput analysis of plasma samples and evaluating the performance of the combined platforms. The conclusions are as follows:
1. Outstanding Performance: The combination of the Proteonano™ Basic Suite and the Evosep one platform demonstrates excellent performance. Plasma samples enriched with the Proteonano™ Plasma Proteome Enrich Kit and processed using both platforms can identify over 2400 proteins, show casing superior protein identification capabilities
2. Increased Depth: The Proteonano™ Basic Suite and Evosep one platform combination offers enhanced coverage, with a dynamic range exceeding 9 orders of magnitude
3.High Standardization: The combined use of the Proteonano™ platform Basic Suite and Evosep one platforms ensures high standardization. Automated control of the entire experimental workflow, including sample preparation,desalting, and mass spectrometry analysis, guarantees the stability of results in large-scale experiments.
In summary, the integration of the Proteonano and Evosep platforms for plasma sample processing provides exceptional throughput and stability for proteomics studies, supporting mass spectrometry-based proteomics for large-scale cohorts of up to thousands of samples.
To learn more, please download the application note.