How to Avoid Pre-Analytical Drivers of Bias in Nanoparticle-Enriched Plasma Proteomics Studies?
Our aim is to prevent inter-batch variability in the Proteonano™ ultraplex proteomics platform that may arise from sample-related factors.
Plasma proteomics is one of the most challenging tasks in clinical proteomics due to the inherent complexity of the samples. Factors such as extreme heterogeneity of proteins at highly dynamic concentrations and improper collection and storage conditions of plasma/serum samples can adversely affect the results and lead to erroneous conclusions. Therefore, it is crucial to evaluate sample quality before initiating a project.
Potential sample related factors include:
➀ Hemolysis
➁ Sample contamination
➂ Duration of sample storage
➃ Pre-treatment methods for plasma samples
➄ Centrifugation
Conclusion
In plasma proteomics experiments, improper sample handling is a major factor contributing to variability between samples. To ensure the highest quality of proteomics data, especially in large-cohort studies, we recommend the following:
1.Avoid contamination from blood cells, platelets, and coagulation factors during sample collection.
2. Prolonged sample storage can lead to nontrivial protein degradaton. We recommend store samples for no more than 3 years, and avoid repeated freeze-thaw cycles.
3. We recommend use the same protocle for plasma sample collection at different sites and complete plasma collection within 8 hours at 4°C.
4. Modest sample (blood) centrifugation speed is 1200 g.
To learn more, please download the application note.