Use of the Proteonano™ Ultraplex platform for deep proteomic studies in mouse plasma samples
To develop a high throughput and reliable workflow that incorporates proteonano™ ultraplex proteomics platform based sample processing to enable deep proteomic analysis of mouse blood samples.
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Mouse models, due to their low cost and ease of access, have become the primary preclinical animal models for studying diseases and drug targets. However, current proteomic workflows for mouse tissues and blood samples face several challenges, including a lack of standardization, inconsistent sample preparation procedures, and variability in data analysis methods. These issues often lead to incomplete data and poor reproducibility, ultimately compromising the accuracy and reliability of experimental results.
The Proteonano™ Ultraplex Proteomics Platform is a highly automated and standardized proteomics sample preparation and analysis system launched by Nanomics Biotech. It consists of the Proteonano™ Kit and the Nanomation™ automated sample processing suite. The Proteonano™ Kit series is developed leverage surface-modified, monodisperse magnetic nanoparticles（ S3MNPs）, and the Nanomation™ automated sample processing suite affords consistent sample processing of large sample cohorts across different sample processing batches. Combination of both components addresses the bottleneck of detecting low-abundance proteins in mass spectrometry in a reliable fashion.
Conclusion
We evaluated the performance the Nanomics Biotech’s Proteonano™ Ultraplex Proteomics Platform for proteomics in different mouse blood samples：
1. The Proteonano™ platform displays high analytical performance: Protein identification results obtained from mouse blood samples processed with the Proteonano™ Mouse Plasma Proteome Enrich Kit and the Proteonano™ Ultraplex Proteomics Platform show a significant improvement compared to direct digestion experiments.
2. The Proteonano™ platform exhibits superior sensitivity: In the comparison of similar products for protein detection in mouse blood samples, the Proteonano™ Mouse Plasma Proteome Enrich Kit stands out for its exceptional protein detection capabilities.
3. The Proteonano™ platform offers high standardization: For different mouse blood samples, an automated, rigorous database (Swiss-Prot) and data analysis process are employed, ensuring the reliability and standardization of the results。
In summary, the Proteonano™ ultraplex platform offers an important technical means for untargeted proteomic analysis of mouse blood samples. This platform demonstrates outstanding accuracy and sensitivity, ensuring an accurate and comprehensive proteomic analysis platform for mouse blood sample analysis and provides a feasible solution for the discovery and translational study of multiple protein biomarkers.
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