Performance of the Proteonano™ Ultraplex Platform on Different Mass Spectrometers
This work is aimed to demonstrate the universality of the proteonano™ultraplex proteomics platform across three major high-resolution mass spectrometers, using low-abundant plasma proteomics as a benchmark.
For years, the field of high-throughput multiplex plasma proteomics has faced critical challenges in achieving reproducible protein quantification with mass spectrometry, limiting its scalability for large-cohort studies. These barriers have slowed the pace of protein biomarker discovery and its clinical translation. Even with major advancements in LC-MS technologies—such as Thermo Fisher’s Orbitrap Astral and Bruker’s timsTOF—the lack of standardized workflows, inconsistent sample preparation, and fragmented data analysis continue to create data silos and hinder reproducibilit.
Nanomics demonstrate the universality of the Proteonano™ Platfrom on three major high resolution mass spectrometers. The goal is to establish standardized analysis procedures for high-throughput plasma proteomics across various mass spectrometers and to offer a practical solution for discovering and translating multiplex protein biomarkers.
Conclusion
This study assesses the performance of the Proteonano™ Ultraplex Proteomics Platform, developed by Nanomics biotechnology, across three high-resolution LC-MS instruments: Orbitrap Exploris480, timsTOF Pro 2, and Orbitrap Astral:
1. This study assesses the performance of the Proteonano™ Ultraplex Proteomics Platform, developed by Nanomics biotechnology, across three high-resolution LC-MS instruments: Orbitrap Exploris 480, timsTOF Pro 2, and Orbitrap Astral
2. Accuracy in protein quantification: the Proteonano™ Platform demonstrates excellent accuracy in protein quantification, with CV values for protein identification below ��% across all three high-resolution mass spectrometers. This ensures data accuracy and highlights the platform’s high sensitivity and reproducibility.
3. High standardization of the Proteonano™ platform: employ automated and rigorous databases (Swiss-Prot) and data analysis workflows across all three mass spectrometers, ensuring reliability and standardization of results.
In summary, the Proteonano™ Ultraplex Proteomics Platform shows outstanding accuracy and sensitivity for plasma proteomics across different mass spectrometry platforms. It establishes standardized analytical processes and provides a practical solution for discovering and translating multiplex protein biomarkers.
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