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Ultraplex Proteomic Analysis of Cerebrospinal Fluid Using the Proteonano™ CSF Proteome Kit

We've developed a standardized cerebrospinal fluid protein analysis workflow using the Proteonano™ Ultraplex Proteomics Platform combined with Orbitrap Astral.

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Proteonano™ Ultraplex Proteomics Platform

Proteonano™️Ultraplex Proteomics Platform workflow

Results of protein groups identified

As the sample volume increases, the number of identified proteins also increases. Even with a minimum sample volume of 100 ng, we were able to identify 1,669 proteins.

Comparison of different CSF Sample Volumes

Abundance range

With sample volumes of 100 ng, 300 ng, and 500 ng using the Orbitrap Astral, we identified a total of 3,114 proteins. Out of these, 2,395 proteins matched entries in the HPPP database, which includes 4,608 proteins. Additionally, concentration information was available for 2,317 of the identified proteins, offering valuable insights into protein levels in CSF samples.

Compare with HPPP database
Proteins dynamic range in CSF sample

Nanomics Biotech

Floor 5, Building 6, AH BioVAlley,

Hangzhou, Zhejiang, China

+86 19357321977

bd@nanomics.bio

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